407143-M

Anti-IP₃ Receptor Rabbit pAb

liquid, Calbiochem^®

• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Espèces réactives: human, canine, mouse, rat, porcine, bovine
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITPR1(3708) ; mouse ... Itpr1(16438) ; rat ... Itpr1(25262)

Description

Description générale

Rabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP₃ receptor protein.

Recognizes all of the ~260 kDa IP₃ receptor subtypes.

This Anti-IP₃ Receptor Rabbit pAb is validated for use in ELISA, FC, Immunoblotting, IF, IHC, IP, Radioimmunoassay for the detection of IP₃ Receptor.

Immunogène

a synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor

Application

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:250)

Immunofluorescence (1:200)

Immunohistochemistry (1:200)

Immunoprecipitation (1:200)

Radioimmunoassay (1:10000)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.

Variables associated with assay conditions will dictate the proper working dilution.



Recommended Protocol for Immunoblotting using anti-IP₃ Receptor Antibodies



NOTE: For protein loading, we recommend 1 x 10⁶ cells/gel lane or 100 μg/gel lane.



• TBST

• 20 mM Tris-HCl, pH 7.6

• 150 mM NaCl

• 0.05% Tween^®-20 detergent



1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).

2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.

3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.

4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.

6. Shake for 1 h.

7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.

8. Mix development solution (1:1) (Amersham ECL kit).

9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable