375230

HGF, Mouse, Recombinant

HGF, Mouse, Recombinant Recombinant, consists of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

• Synonyme(s) : Hepatopoietin A
• Code UNSPSC : 12352203

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (as seen by a 60 kDa (α chain) and a 33 kDa (β chain) bands under reducing conditions., SDS-PAGE)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: <1.0 EU/μg protein Endotoxin
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Recombinant, mouse hepatocyte growth factor (HGF) consisting of disulfide-linked heterodimeric α (Gln33 to Arg495) and β (Val496 to Leu728) chains expressed in murine myeloma NS0 cell line.

Actions biochimiques/physiologiques

Cell Proliferation:

ED₅₀ = 10-30 ng/ml as measured in a cell proliferation assay using IMCD-3 mouse epithelial cells.



Functional ELISA

K_D<0.2 nM as measured by binding to C-Met (HGF Receptor).

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Autres remarques

Corso, S., et al. 2005. Trends Mol. Med.11, 284.
Karihaloo, A., et al. 2005. Nephron Exp. Nephrol.100, e40.
Urbanek, K., et al. 2005. Circ. Res.97, 663.
Hammond, D.E., et al. 2004. Curr. Top. Microbiol. Immunol.286, 21.
Rosario, M. and W. Birchmeier. 2004. Dev. Cell7, 3.
Gheradi, E., et al. 2003. Proc. Natl. Acad. Sci.100, 12039.
Chiu, S.J., et al. 2002. J. Biomed. Sci.9, 261.
Tanaka, Y., et al. 2002. Biochem. Biophys. Res. Commun.299, 472.
Maeshima, A., et al. 2000. Kid. Int.58, 1511.
Lesk, A.M. and W.D. Fordham. 1996. J. Mol. Biol.258, 501.
Saelman, E.U.M., et al. 1995. J. Cell Sci.108, 3531.
Mineo, R., et al. 1994. Endocrinology145, 4355.
Mizuno, K., et al. 1994. J. Biol. Chem.269, 1131.
Sasaki, M., et al. 1994. Biochem. Biophys. Res. Commun.199, 772.
Montesano, R., et al. 1991. Cell67, 901.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3