218829

Caspase-3 Substrate IX, Fluorogenic

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate.

• Synonyme(s) : Caspase-3 Substrate IX, Fluorogenic, (Z-DEVD)₂-Rh 110
• Formule empirique (notation de Hill): C₇₂H₇₈N₁₀O₂₇
• Poids moléculaire : 1515.44
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Essai: ≥95% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: yellow
• Solubilité: DMSO: 1 mg/mL
• Fluorescence: λ_ex ~485 nm; λ_em ~535 nm
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photo-stable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Also reported to detect capase-3 activity in intact cells by flow cytometry. Useful for high throughput screening applications. Excitation max: ~485 nm; emission max: ~535 nm.

A non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide that acts as a highly sensitive, photostable caspase-3 fluorogenic substrate. Reported to detect less than 1 ng/ml of caspase-3. Useful for high throughput screening applications.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
A highly sensitive, photostable caspase-3 fluorogenic substrate

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Séquence

(Z-Asp-Glu-Val-Asp)₂-Rhodamine 110

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Hug, H., et al. 1999. Biochemistry38, 13906.
Liu, J., et al. 1999. Bioorg. Med. Chem. Lett.9, 3231.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable