218765

Caspase-9 Substrate I, Fluorogenic

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

• Synonyme(s) : Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
• Formule empirique (notation de Hill): C₃₃H₃₈F₃N₇O₁₁
• Numéro CAS: 210345-03-2
• Poids moléculaire : 765.69
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: white
• Solubilité: DMSO: 5 mg/mL
• Fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore (excitation maximum: ~400 nm; emission maximum: ~505 nm).

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Séquence

Ac-Leu-Glu-His-Asp-AFC

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable