09-822

Anti-KLF5 (Krüppel-like factor 5) Antibody

serum, from rabbit

• Synonyme(s) : BTE-binding protein 2, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC box binding protein 2, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Kruppel-like factor 5, Kruppel-like factor 5 (intestinal), Tra
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse
• Technique(s): ChIP: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_033899
• Numéro d'accès UniProt: Q9Z0Z7
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KLF5(688)

Description

Description générale

Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation.

Spécificité

This antibody recognizes Krüppel-like factor 5 at the N-terminus.

Immunogène

Epitope: N-terminus

Recombinant protein corresponding to mouse Krüppel-like factor 5 at the N-terminus.

Application

Anti-KLF5 (Krüppel-like factor 5) Antibody is a Rabbit Polyclonal Antibody for detection of KLF5 (Krüppel-like factor 5) also known as Krüppel-like factor 5 & has been validated in WB.

Chromatin Immunoprecipitation Analysis (ChIP):
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Pluripotent & Early Differentiation

Qualité

Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.

Description de la cible

~55 kDa observed. An uncharacterized band appears at ~30 kDa

Forme physique

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Mouse embryonic stem cell chromatin extract

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1