05-1313
Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
clone HWA4C4, from mouse
Synonyme(s) :
ubiquitin C, polyubiquitin B, ubiquitin B
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
HWA4C4, monoclonal
Espèces réactives
vertebrates, human
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... UBAP2(55833)
Description générale
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
Spécificité
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Immunogène
Epitope: Lys63 ubiquitination site
Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).
Application
Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
Research Category
Signaling
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications
Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.
Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer′s patient.
ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications
Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.
Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer′s patient.
ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.
Qualité
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Description de la cible
varies
Forme physique
Format: Purified
Purified immunoglobulin by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Remarque sur l'analyse
Control
Lys63 polyubiquitin chains.
Lys63 polyubiquitin chains.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein.
Galan, J M and Haguenauer-Tsapis, R
The Embo Journal, 16, 5847-5854 (1997)
Lys63-linked polyubiquitin chains: linking more than just ubiquitin.
Kawadler, Holli and Yang, Xiaolu
Cancer Biology & Therapy, 5, 1273-1274 (2006)
Xingguang Liu et al.
Cell death and differentiation, 24(4), 683-693 (2017-02-06)
Lung ischemia-reperfusion (I/R) injury remains one of the most common complications after various cardiopulmonary surgeries. The inflammation response triggered by the released damage-associated molecular patterns (DAMPs) aggravates lung tissue damage. However, little is known about the role of autophagy in
Jun Meng et al.
Cell death & disease, 8(6), e2866-e2866 (2017-06-09)
Enterovirus 71 (EV71) is the main causative agent of hand, foot and mouth disease (HFMD), which induces significantly elevated levels of cytokines and chemokines, leading to local or system inflammation and severe complications, whereas the underlying regulatory mechanisms and the
I-Ting Chen et al.
Scientific reports, 5, 12300-12300 (2015-07-21)
Toll-like receptor 4 (TLR4) plays an important role in innate immunity by eliciting inflammation. Upon receptor engagement, transforming growth factor β-activated kinase 1 (TAK1) is an essential mediator that transmits a signal from the receptor to downstream effectors, IκB kinase
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