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G004731

HPLC Analysis of Cresols and Phenol on Astec® CYCLOBOND I 2000

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase: [A] water; [B] methanol; (90:10, A:B)
flow rate: 1 mL/min
column temp.: 22 °C
detector: UV, 254 nm
injection: 1 μL
sample: 0.5 mg/mL in methanol

peak 1: o-cresol (10.70 min)

peak 2: phenol (11.70 min)

peak 3: m-cresol (14.20 min)

peak 4: p-cresol (20.10 min)

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    o-Cresol, ≥99%
    Phenol, for molecular biology
    m-Cresol, 99% (GC)
    p-Cresol, ≥99%, semisolid

analytical column

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Description
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