U8501
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast
Type X, lyophilized powder, ≥40 units/mg protein
Synonym(s):
UTP:α-D-glucose-1-phosphate uridylyltransferase
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About This Item
Recommended Products
biological source
bakers yeast
Quality Level
type
Type X
form
lyophilized powder
specific activity
≥40 units/mg protein
composition
Protein, 30-60% modified Warburg-Christian
foreign activity
UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%
storage temp.
−20°C
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General description
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.
Application
Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
- in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9
- to quantify Suc synthase (SUS) enzyme activity in rice
- to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes
Biochem/physiol Actions
Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.
Unit Definition
One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.
Physical form
Lyophilized, sulfate-free powder containing citrate buffer salt
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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