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T9179

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Terrific Broth

EZMix powder microbial growth medium

Synonym(s):

TB media

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

Quality Level

200

description

TB; quick dissolve, dust-free formulation

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
  • as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
  • to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
  • Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)

Features and Benefits

Terrific Broth EZMix powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • A budget-friendly alternative to liquid
  • Modified formulation (without glycerol)
  • Easy scale-up using larger package sizes

Components

*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4

Reconstitution

Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lac V Lee et al.
Biochemistry, 49(25), 5366-5376 (2010-05-19)
Undecaprenyl pyrophosphate synthase (UPPS) catalyzes the consecutive condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to generate the C(55) undecaprenyl pyrophosphate (UPP). It has been demonstrated that tetramic acids (TAs) are selective and potent inhibitors of
Cynthia Sanville Millard et al.
Protein expression and purification, 29(2), 311-320 (2003-05-28)
Contemporary approaches to biology often call for the high-throughput production of large amounts of numerous proteins for structural or functional studies. Even with the highly efficient protein expression systems developed in Escherichia coli, production of these proteins is laborious and
Optimizing antibody immobilization strategies for the construction of protein microarrays
Peluso P, et al.
Analytical Biochemistry, 312(2), 113-124 (2003)
Paul Peluso et al.
Analytical biochemistry, 312(2), 113-124 (2003-01-18)
Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized
Idan Tuval et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(7), 2277-2282 (2005-02-09)
Aerobic bacteria often live in thin fluid layers near solid-air-water contact lines, in which the biology of chemotaxis, metabolism, and cell-cell signaling is intimately connected to the physics of buoyancy, diffusion, and mixing. Using the geometry of a sessile drop
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Protocols

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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