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T9039

Sigma-Aldrich

Tris Buffered Saline, with Tween™ 20, pH 8.0

Tris Buffered Saline, with Tween™ 20, pH 8.0

powder, pH 8.0, with Tween® 20

Synonym(s):

TBS, TBST

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10 PKG
CHF 156.00

CHF 156.00


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Diagnostics Manufacturers should use alternative grade product: SRE0031

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10 PKG
CHF 156.00

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

CHF 156.00


Check Cart for Availability
Diagnostics Manufacturers should use alternative grade product: SRE0031

Request a Bulk Order

Product Name

Tris Buffered Saline, with Tween™ 20, pH 8.0, powder

form

powder

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Application

Tris Buffered Saline, with Tween® 20, pH 8.0 has been used as a wash buffer in western blotting and enzyme linked immunosorbent assay.[1][2][3] It has also been used along with 5% milk to block proteins on nitrocellulose in western blotting.[4]

Biochem/physiol Actions

Tris Buffered Saline with Tween 20 is predominantly used as a wash buffer[5] and also in blocking steps of immunoblotting technique.[6]

Packaging

Foil pouches

Reconstitution

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.05 M Tris buffered saline (NaCl - 0.138 M; KCl - 0.0027 M); TWEEN® 20 - 0.05%, pH 8.0, at 25 °C.

Legal Information

TWEEN is a registered trademark of Croda International PLC

related product

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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