Journal of chromatography. A, 720(1-2), 235-249 (1996-01-12)
N-linked oligosaccharides from glycoproteins can be either analyzed on a sub-nanomole scale or preparatively purified on a multi-micromole scale. Each goal necessitates a unique analytical strategy often involving oligosaccharide derivatization to enhance separation and detection. Tyrosinamide-oligosaccharides were developed to facilitate
The N-linked oligosaccharide from soy bean agglutinin (Man9) was isolated on a preparative scale following derivatization with Boc-tyrosine. The procedure utilized preparative hydrazinolysis to release the oligosaccharide and yielded multi-micromol quantities of Boc-tyrosine-Man9 which was characterized by 1H NMR and
Archives of biochemistry and biophysics, 318(2), 465-475 (1995-04-20)
A library of 15 N-linked oligosaccharide structures was prepared from ovalbumin and characterized using high-field NMR and mass spectrometry. The oligosaccharides were enzymatically released from ovalbumin glycopeptides, and the reducing ends were reacted with ammonium bicarbonate to form oligosaccharide-glycosylamines. These
A novel approach to 14C label N-linked oligosaccharides.
In a previous report (Tamura, T., Wadhwa, M.S., and Rice, K.G. (1994) Anal. Biochem. 216, 335-344) we described the derivatization of N-linked oligosaccharides with Boc-tyrosine resulting in the formation of tyrosinamide-oligosaccharides. Attachment of Boc-tyrosine to the reducing-end of an oligosaccharide
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