SRP4542

FGF-19 human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

• Synonym(s): FGF19, FGFJ, fibroblast growth factor 19
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• potency: 50-200 ng/mL
• mol wt: ~21.8 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: NM_005117
• UniProt accession no.: O95750
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FGF19(9965)

Description

General description

Fibroblast growth factor-19 (FGF-19) is an ileum-derived polypeptide hormone and a high affinity heparin-dependent ligand for fibroblast growth factor receptor 4 (FGFR4). It is part of the hormone-like FGF family. The gene encoding FGF-19 is localized on human chromosome 11q13.3. Recombinant human FGF-19 is a 21.8kDa protein containing 195 amino acid residues.

Biochem/physiol Actions

Fibroblast growth factor-19 (FGF-19) is involved in the gut-liver signaling pathway and modulation of bile acid synthesis. Once secreted, it binds to the hepatic fibroblast growth factor receptor 4 (FGFR4)/Klotho-β cell-surface receptor complex and activates it. FGF-19 promotes glycogen synthesis, protein synthesis and lipid metabolism.

Physical form

Lyophilized from 5 mM Tris, pH 7.6 plus 75 mM NaCl. The protein may appear as a haze or film, which you may not see at the bottom of the vial.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1 to 1.0 μg/μL. This solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable