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S7651

Sigma-Aldrich

Sphingomyelinase from Bacillus cereus

lyophilized powder, ≥100 units/mg protein

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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10 UNITS
CHF 99.60
50 UNITS
CHF 310.00

CHF 99.60

Estimated to ship on13 April 2025

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10 UNITS
CHF 99.60
50 UNITS
CHF 310.00

About This Item

CAS Number:
9031-54-3
Enzyme Commission number:
3.1.4.12 (BRENDA, IUBMB)
EC Number:
232-869-9
MDL number:
MFCD00132362
UNSPSC Code:
12352204
NACRES:
NA.54

CHF 99.60

Estimated to ship on13 April 2025

form

lyophilized powder

Quality Level

200

specific activity

≥100 units/mg protein

mol wt

36873 Da

composition

Protein, ~10% Lowry

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 14579 ... BC0671(1203022)

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General description

Sphingomyelinase from Bacillus cereus (Bc-Smase) is a Mg2+-containing metalloenzyme.[1] Bacillus cereus sphingomyelinase induces hemolysis of sheep erythrocytes.[2]
Sphingomyelinase modulates adenosine triphosphate (ATP)-independent endocytosis of fibroblast and large vesicles of macrophages.[3] Sphingomyelinase causes hemolysis of human erythrocytes. It is also a potent virulence factor leading to invasive diseases.[4]

Application

Sphingomyelinase from Bacillus cereus has been used:
  • in the measurement of membrane capacitance in brain slices[3]
  • for testing and confirming its silkworm-killing activity[4]
  • in enzyme activity assay and for elucidating its effect on hydrolysis of sphingomyelin in complex lipid mixtures[5]

Sphingomyelinase has been used in a study to investigate skeletal manifestations in pediatric and adult patients with Niemann Pick disease. [6]

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Unit Definition

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000287995
0000287995
0000220532
0000220311
0000220532

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Masataka Oda et al.
Biochimica et biophysica acta, 1798(6), 1073-1080 (2010-03-11)
Bacillus cereus sphingomyelinase (Bc-SMase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. We investigated the mechanism of this hemolysis in comparison to that induced by Clostridium perfringens alpha-toxin. Pertussis toxin, a Gi-specific inhibitor, N-oleoylethernolamine, a ceramidase inhibitor
Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity
Usui K, et al.
Microbial Pathogenesis, 46(2), 59-62 (2009)
Lipid bilayers containing sphingomyelins and ceramides of varying N-acyl lengths: a glimpse into sphingolipid complexity
Jimenez-Rojo N, et al.
Biochimica et Biophysica Acta - Biomembranes, 1838(1), 456-464 (2014)
Masataka Oda
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 129(10), 1233-1238 (2009-10-03)
Bacillus cereus is one that causes of opportunistic human infections. Sphingomyelinase produced by B. cereus is assumed a virulence factor for the infection. Sphingomyelinase from Bacillus cereus (Bc-SMase) is Mg(2+)-containing metalloenzyme. Bc-SMase is a family of neutral SMase (nSMase) and
Melissa Wasserstein et al.
Journal of inherited metabolic disease, 36(1), 123-127 (2012-06-22)
Niemann-Pick disease (NPD) due to acid sphingomyelinase deficiency is a lipid storage disease resulting from the accumulation of sphingomyelin, predominantly within cells of the monocyte-macrophage system. In contrast to other lysosomal storage disorders, skeletal involvement in NPD has not been
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Questions

  1. Hi for product number S7651, Sphingomyelinase from Bacillus cereus, once prepared how long can the Sphingomyelinase solution be kept and should it be kept at 2-8C or -20C?

    1 answer

    1. This product is stable for several days when stored refrigerated. Prepared solutions in aqueous buffers containing 50 % Glycerol are stable for extended periods when refrigerated.

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