S2454
Seppro™ Ammonium Bicarbonate Buffer
Synonym(s):
ABC buffer, Ammonium hydrogen carbonate, Monoammonium carbonate
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.32
Recommended Products
form
solution
Quality Level
concentration
2 M
technique(s)
protein expression: suitable
color
clear colorless
application(s)
life science and biopharma
storage temp.
room temp
General description
Seppro® Ammonium Bicarbonate Buffer is an effective buffer for protein digestion and deglycosylation reaction. It may be used to coat 2D and 3D electrophoretic gel pieces during digestion of proteins and proteomes. The ammonium bicarbonate buffer also provides moisture during enzymatic cleavage. Ammonium bicarbonate buffer regulates the microenvironmental pH of immobilized cephalosporin C acylase (CCA) for the catalysis of cephalosporin C (CPC) in a batch stirred tank reactor (STR).
Application
Ammonium bicarbonate buffer has been used as a component of cell lysis/protein extraction buffer for proteomic sample preparation.
Legal Information
6538 is a trademark of American Type Culture Collection
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Yanhong Chang et al.
Biotechnology progress, 35(5), e2846-e2846 (2019-05-18)
In a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH dropped to 7.2 in a nonbuffered system (with the pH maintained at 8.5 by adding alkali) due to the existence of diffusional resistance. Moreover
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Andrej Shevchenko et al.
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In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel
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