The fluorophore has excitation at 520 nm and emission at 605 nm which is important for determining fluorescence intensity.
MAK145
Fluorometric Intracellular Ros Kit
sufficient for 200 fluorometric tests (red)
Synonym(s):
Intracellular ROS Fluorescence Assay
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.25
Recommended Products
usage
sufficient for 200 fluorometric tests (red)
detection method
fluorometric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
General description
Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell. At physiological levels, ROS contribute to cell signaling and host defense.[1] Increased ROS generation, above the detoxification capacity of the biological system, results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[2]
Application
Suitability
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader or a fluorescent microscope.
Principle
The kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radicals) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 520/λem = 605 nm) proportional to the amount of ROS present The assay can be performed in either a 96 or 384 multiwell plate format using a fluorescence microplate reader.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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