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MAK130

Sigma-Aldrich

Chromium Assay Kit

sufficient for 250 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 250 colorimetric tests

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Chromium, an essential trace element, is a transition metal that primarily exists in two stable oxidation states, hexavalent [Cr(VI)] and trivalent [Cr(III)]. Chromium is widely used in various industries such as electroplating, leather tanning, chrome paint, dying, hardened steel, ceramic, and glass. Cr(VI) is produced almost exclusively by industrial processes; whereas, in nature chromium primarily exists in its trivalent form. Cr(III) is generally regarded as nontoxic due to its poor absorption. Cr(VI) is considered a pulmonary carcinogen and has tested positive in genotoxicity tests. Cr(VI) is considered a serious pollutant in many water streams due to its carcinogenic potential. In trace amounts, Cr(III) may be required for glucose and lipid metabolism.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

This kit is suitable for the detection of chromium in biological, environmental, food, and beverage samples.

Principle

In this kit, chromium is determined in a simple, one-step assay in which Cr(VI) forms a specific complex with a chromgenic dye, resulting in a colorimetric (480 nm) product, proportional to the chromium present. Cr(III) can be converted to Cr(VI) with nitric acid/hydrochloric acid, allowing the determination of Cr(III) and/or total chromium [Cr(III) +Cr(VI)].

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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