L9639
Ricin B chain from Ricinus communis (castor bean)
buffered aqueous solution
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About This Item
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form
buffered aqueous solution
storage temp.
2-8°C
Biochem/physiol Actions
Ricin B chain has an affinity for galactose and saccharides containing nonreducing terminal β-galactosyl units. This affinity allows the B chain to bind to cell surface glycoconjugates. In the intact ricin toxin, the surface binding by the B chain allows the A chain to enter into the cell. The saccharide binding capability of ricin B chain is independent of the presence of the A chain.
Other Notes
Ricinus communis toxin (also named RCA60, RCAII, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.
Quality
Electrophoretically pure (SDS-PAGE)
Physical form
Solution in 10 mM phosphate, pH 6.5, containing 0.15 M NaCl, 10 mM galactose, 0.5 mM dithioerythritol and 0.02% sodium azide
Preparation Note
Highly purified by affinity chromatography
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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