The pH of the media can be 7.0, but depending on the application, it can range from 7.0 to 8.0. The pH can be adjusted using 1N NaOH and 1N HCl.
L7275
LB Broth (Lennox)
LB Broth (Lennox)
Tablet microbial growth medium
Synonym(s):
Lennox broth , Luria Bertani Broth
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100 TABS
CHF 123.00
500 TABS
CHF 503.00
CHF 123.00
Estimated to ship on10 April 2025
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100 TABS
CHF 123.00
500 TABS
CHF 503.00
About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
CHF 123.00
Estimated to ship on10 April 2025
Recommended Products
grade
for molecular biology
Quality Level
sterility
non-sterile
form
tablet
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
Dehydrated Luria-Bertani (LB) broth is called the Lennox LB, which has amino acids as carbon source.[1]
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
Application
LB Broth (Lennox) has been used for culturing Escherichia coli[2][3] and bacterial artificial chromosomes (BACs).[4]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Lennox LB tablets provide:
- Convenient small package to eliminate weighing
- Fast preparation – no stirring required
Components
10 g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
5 g/L Yeast Extract
5 g/L NaCl
2.2 g/L inert binding agents
Reconstitution
Add each 1.1g tablet in 48.3mL water (no stirring required). Autoclave for 20 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Legal Information
Zeocin is a registered trademark of Cayla Sarl
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
General protocols for growth of competent cells in microbial medium.
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Do the tablets contain any additives to adjust the pH of the media? Also, what should the final pH of the media be?
1 answer-
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What is the difference among the many LB - Lennox products?
1 answer-
LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
Helpful?
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
Helpful?
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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