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Key Documents

L5293

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4

Ready Made solution, 1 mg/mL

Synonym(s):

LPS

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2 ML
CHF 357.00

CHF 357.00

Estimated to ship on08 April 2025

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2 ML
CHF 357.00

About This Item

MDL number:
MFCD00164401
UNSPSC Code:
12352201
NACRES:
NA.25

CHF 357.00

Estimated to ship on08 April 2025

Request a Bulk Order

biological source

Escherichia coli (O111:B4)

Quality Level

200

form

aqueous solution

concentration

1 mg/mL

color

colorless to faint yellow

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

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Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Lipopolysaccharides from Escherichia coli O111:B4 has been used:
  • to stimulate an inflammatory response in porcine liver tissues in-vivo[1] and piglet ileum tissues ex vivo[2]
  • to stimulate Ex vivo natural killer(NK) cells[3] and hepatic B cells[4]
  • to induce arthritis in a standard rabbit model[5]

Biochem/physiol Actions

Lipopolysaccharides (LPS) are distributed on the outer layer of the membrane and in non-capsulated strains, present on the cell surface. LPS maintains the outer membrane integrity. In addition, it protects the cell from bile salts stress and lipophilic antibiotics.[6]

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000408042
0000408042
0000294757
0000212837
0000219279

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Effects of dietary beta-glucans supplementation on cytokine expression in porcine liver
Ryan M, et al.
Journal of Animal Science, 90(suppl-4), 40-42 (2012)
Imaging of normal and pathologic joint synovium using nonlinear optical microscopy as a potential diagnostic tool
Tiwari N, et al.
Journal of Leukocyte Biology, 15(5), 056001-056001 (2010)
6 Analysis of Lipopolysaccharides of Gram-Negative Bacteria
Methods in Microbiology, 18(5), 157-207 (1985)
Ryan J Lu et al.
Nature aging, 1(8), 715-733 (2021-09-14)
Neutrophils are the most abundant human white blood cell and constitute a first line of defense in the innate immune response. Neutrophils are short-lived cells, and thus the impact of organismal aging on neutrophil biology, especially as a function of
NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection
Wesselkamper S, et al.
Journal of immunology (Baltimore, Md. : 1950), 181(8), 5481-5489 (2008)
View All Related Papers

Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Related Content

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is recommended for the storage of longer duration once opened? Is it better to keep at 2°C-8°C for 1-2 years or to freeze it (-80°C or -20°C) and thaw it gently when needed?

    1 answer

    1. This product is stable at 2-8°C at least until the assigned retest date, provided that no contaminants have been introduced. The recommended retest date is lot specific and reported in the product Certificate of Analysis. The stability of the product in dilute form or beyond the assigned retest date has not been determined. However, LPS products prepared from the powder form may be stored in 1 mg/mL aliquots for up to 2 years at -20°C. The storage of solutions at -80°C has not been determined. Please see the link below to review additional information regarding the stability and preparation of Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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  4. What solvent is the product L5293-2ML dissolved in?

    1 answer

    1. The solvent information for this product is proprietary, but it is acknowledged to contain water, while the remaining components remain undisclosed.

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  5. Bonjour, Comment ce LPS a t'il été extrait / Purifié ?

    1 answer

    1. This product is a sterile filtered solution. The extraction method for this material is considered proprietary, however it is chromatographically purified.

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