Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

I5036

Sigma-Aldrich

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in E. coli, lyophilized powder, ≥80 units/mg protein

Synonym(s):

IDH1, Isocitrate Dehydrogenase Cytoplasmic

Sign Into View Organizational & Contract Pricing

Select a Size

100 μG
CHF 533.00

CHF 533.00

Estimated to ship on13 April 2025

All Photos(1)

Select a Size

Change View
100 μG
CHF 533.00

About This Item

CAS Number:
9028-48-2
Enzyme Commission number:
1.1.1.42 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

CHF 533.00

Estimated to ship on13 April 2025

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥80 units/mg protein

mol wt

46,658 Da

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Isocitrate dehydrogenase 1 (IDH1) gene is located on human chromosome 2q33. IDH1 is present in the cytoplasm and peroxisomes.[1] It is expressed at high levels in the mammalian liver. IDH1 consists of a C-terminal tripeptide peroxisome, that targets the signal one sequence.[2]

Biochem/physiol Actions

Isocitrate dehydrogenase 1 (IDH1) help in the oxidative carboxylation of isocitrate to α-ketoglutarate, that results in the formation of reduced nicotinamide adenine dinucleotide phosphate (NADPH).[1] It participates in lipid metabolism and glucose sensing. Suppressing IDH1 in pancreatic islets affects the insulin secretion, induced by glucose and leads to increase in lactate production.[2]

Unit Definition

One unit corresponds to the amount of enzyme which converts 1.0 μmol DL-isocitrate to α-ketoglutarate per minute at pH 7.4 and 37 °C (NADP as cofactor).

Physical form

Supplied as a lyophilized powder containing Tris-HCl pH 8.0, trehalose, ammonium sulfate and DTT

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000221530
0000221530
0000088411
11130909
093M4064V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Isocitrate dehydrogenase 1 codon 132 mutation is an important prognostic biomarker in gliomas
Sanson M, et al.
Journal of Clinical Oncology, 27(25), 4150-4154 (2009)
Mast cell tryptase and carboxypeptidase A expression in body fluid and gastrointestinal tract associated with drug-related fatal anaphylaxis
Guo X J, et al.
World Journal of Gastroenterology, 21(47), 13288-13288 (2015)
Theresa Schumacher et al.
Nature, 512(7514), 324-327 (2014-07-22)
Monoallelic point mutations of isocitrate dehydrogenase type 1 (IDH1) are an early and defining event in the development of a subgroup of gliomas and other types of tumour. They almost uniformly occur in the critical arginine residue (Arg 132) in the
Liran I Shlush et al.
Nature, 506(7488), 328-333 (2014-02-14)
In acute myeloid leukaemia (AML), the cell of origin, nature and biological consequences of initiating lesions, and order of subsequent mutations remain poorly understood, as AML is typically diagnosed without observation of a pre-leukaemic phase. Here, highly purified haematopoietic stem
Waraporn Chan-On et al.
Nature genetics, 45(12), 1474-1478 (2013-11-05)
The impact of different carcinogenic exposures on the specific patterns of somatic mutation in human tumors remains unclear. To address this issue, we profiled 209 cholangiocarcinomas (CCAs) from Asia and Europe, including 108 cases caused by infection with the liver
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service