HLTUD0262

MISSION^® Lenti microRNA Inhibitor, Human

hsa-miR-495-3p

• Synonym(s): Tough Decoy, TuD
• UNSPSC Code: 41106609
• NACRES: NA.51

Properties

• Quality Level: 200
• product line: MISSION^®
• form: liquid
• concentration: ≥1x10⁶ VP/ml (via p24 assay)
• technique(s): capture ELISA: 10⁶ TU/mL using p24 (Volume 200 uL)
• mature sequence: AAACAAACAUGGUGCACUUCUU
• Sanger mature/minor accession no.: MIMAT0002817
• storage temp.: −70°C

Description

General description

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. miRNA are known to regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. The lentiviral microRNA Inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element2 (WPRE) is included, allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells.

• Allows for potent inhibition of the desired miRNA
• Lentiviral delivery format allows for efficient delivery of the inhibitor into a wide variety of cell types
• Enables long-term inhibition without repeat transfection

Other Notes

Based on miRBase V19 Mature ID

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable