H8280
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow
matrix fast-flow highly cross-linked 4% beaded agarose
Synonym(s):
N-Hydroxysuccinimidyl-Agarose
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About This Item
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form
isopropanol suspension
extent of labeling
16-23 μmol per mL
technique(s)
protein array: suitable
matrix
fast-flow highly cross-linked 4% beaded agarose
matrix active group
N-hydroxysuccinimide active ester
matrix spacer
6 atoms (when ligands are coupled by displacement of NHS)
storage temp.
2-8°C
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General description
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow is a cross-linked and preactivated matrix obtained by mixing Sepharose 4 Fast Flow with 6-aminohexanoic acid through a spacer arm. Esterification with N-hydroxysuccinimide activates the terminal carboxyl group. Ligand-containing primary amino groups directly interact with this active ester to form a chemically very stable amide linkage.
Application
N-Hydroxysuccinimidyl-Sepharose™ 4 Fast Flow has been used as a part of a magnetic particles-based visual detection system to achieve separation upon binding of the amplified gene. It has also been used to immobilize protein during pull-down assay.
Features and Benefits
- Specifically recommended for coupling of small amino-containing proteins and peptides in process-scale applications
- High level of pre-activation, which gives a high degree of substitution of the selected ligand
- NHS coupling method enables chemically stable ligand attachment
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
53.6 °F
Flash Point(C)
12 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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