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GW23153

Sigma-Aldrich

Anti-Rubisco (plant) antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

chicken

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

plant (spinach)

manufacturer/tradename

Genway 15-288-23153

technique(s)

western blot: suitable

NCBI accession no.

AAC29137.1

UniProt accession no.

Q9TIM3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

spinach ... rbcL(2715621)

General description

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is an enzyme wth a molecular weight of 560 kDa.

Immunogen

Immunogen Sequence: GI # 3403236, sequence 1-491
Rubisco (plant).

Application

Anti-Rubisco (plant) antibody produced in chicken has been used to detect Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) by immunoblotting.

Biochem/physiol Actions

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is involved in catalyzing the addition of CO2 to ribulose-1,5-bisphosphate (RuBP). It is involved in the first step of photosynthesis, that results in the net gain of atmospheric CO2 to the biosphere. Rubisco possesses oxygenase activity. Mutation of rubisco influences photorespiration and plant growth.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Directed mutation of the Rubisco large subunit of tobacco influences photorespiration and growth
Whitney S, et al.
Plant Physiology, 121(2), 579-588 (1999)
Rubisco: structure, regulatory interactions, and possibilities for a better enzyme
Spreitzer R J, et al.
Annual Review of Plant Biology, 53 (2002)
Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks
Liu K H, et al.
Nature, 545(7654), 311-311 (2017)
First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii
Taylor T C, et al.
Test, 276(51), 48159-48164 (2001)
Kun-Hsiang Liu et al.
Nature, 545(7654), 311-316 (2017-05-11)
Nutrient signalling integrates and coordinates gene expression, metabolism and growth. However, its primary molecular mechanisms remain incompletely understood in plants and animals. Here we report unique Ca2+ signalling triggered by nitrate with live imaging of an ultrasensitive biosensor in Arabidopsis

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