F4055

Anti-FMR1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-FMRP, Anti-Fragile X Mental Retardation Protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: human, mouse, rat
• enhanced validation: functional assay; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate; indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells; western blot: 1-2 μg/mL using HEK-293T cell lysate; western blot: 2-4 μg/mL using RAT1 cell lysate
• UniProt accession no.: Q06787
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

Description

General description

FMR1 is a RNA binding protein expressed mainly in brain, neurons, placenta, testes and lymphocytes. Defect in FMR1 can lead to Fragile X Mental Retardation Syndrome due to lack of expression of FMR1 or expression of a mutant protein that cannot bind RNA. Anti-FMR1 (C-terminal) antibody can be used in immunofluorescence staining. Rabbit anti-FMR1 (C-terminal) antibody reacts specifically with FMR1.

The FMR1 protein (fragile X mental retardation 1 protein) can bind to RNA. It contains two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one RGG box. Two proteins named FXR1 and FXR2 interact with FMR1. The protein is highly expressed in brain and testis.

Immunogen

synthetic peptide corresponding to amino acids 606-623 of human FMR1, conjugated to KLH. The corresponding sequence is highly conserved (1 amino acid substitution) in rat and mouse.

Application

Anti-FMR1 (C-terminal) antibody produced in rabbit has been used in: western blotting, immunoprecipitation, immunofluorescence, immunoblotting .

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves