This product has not been tested for any specific application, including in vivo use. Suitability would have to be determined experimentally by the end user. Historical information suggests that a concentration of 0.5% Evans Blue in PBS at pH 7.4 has been used successfully in staining tissue sections. This concentration may also be effective starting point for in vivo applications. Please refer to available literature for additional information.
E2129
Evans Blue
Dye content ≥75 %, Powder
Synonym(s):
Direct Blue 53
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10 G
CHF 99.30
50 G
CHF 398.00
100 G
CHF 585.00
About This Item
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Colour Index Number:
23860
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
Product Name
Evans Blue, Dye content ≥75 %
form
powder
Quality Level
composition
Dye content, ≥75%
technique(s)
titration: suitable
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1\N=N\c2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(\N=N\c5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
InChI key
ATNOAWAQFYGAOY-GPTZEZBUSA-J
Gene Information
human ... PTPN1(5770) , PTPRF(5792)
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General description
Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.
Application
Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.
Biochem/physiol Actions
Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability.[1] It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.[2]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What amount and concentration should I use Evans-Blaun injecting it into the ear skin of a mouse to see Evans-Blue in the draining Lymphnodes afterwards?
1 answer-
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What is the stability of the E2129 solution once prepared?
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The stability of the solution will depend on whether the solutions are sterile filtered or contaminated through routine use. It's recommended to store the solutions in the dark at 2-8°C.
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How to prepare a stock 1% solution using E2129, and is ther any guidance on its use?
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A 0.5% solution of Evans Blue in PBS, pH 7.4. Product P3813 should be used as the PBS, which will serve as the stock solution. For use, 3-4 drops should be added to 100 mL of PBS P3813. This should be used as the final rinse with a 5-minute wash. After rinsing briefly, coverslip the sample.
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Should Evans Blue solutions for fluorescent assays be aged or cured for 7 days before use?
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While some dyes require aging before use, there is no indication that Evans Blue solutions need to be aged or cured before use.
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How do I dilute 80% purity evans blue powder into a 2% solution with normal saline?
1 answer-
This product is soluble in water at 60 mg/mL. For typical laboratory staining applications, a 1X PBS solution is also commonly used. Solubility in normal saline has not been evaluated, but rates are expected to be similar. A 2% solution would be 20 mg/mL. The dye content is lot-specific and reported in the Certificate of Analysis. Please see the link below to access a sample or batch-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/e2129#product-documentationHelpful?
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What is the difference between Evans Blue Products E2129 and 206334?
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Both products are Evans Blue. Product No. E2129 has a dye content of 80% and Product No. 206334 has a dye content of approximately 85%.
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How should Product E2129, Evans Blue, be dissolved?
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This product is soluble in water, alcohol, acids and alkalies.
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Can Evans Blue, Product E2129, be used as a counterstain for FITC?
1 answer-
This product can be used as a counterstain in immunohistochemistry when using FITC. After staining for immunofluorescence, dip sections in a 0.1% (w/v) in water solution of Evans Blue for 5-10 minutes. Rinse well in fresh PBS or water before coverslipping. Reference: Immunocytochemistry, Theory and Practice, p. 82 (1988).
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What is the shelf life of Product E2129, Evans Blue?
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This product has a retest date of 4 years. This information can be found on the Certificate of Analysis.
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What is the solution stability of Product E2129, Evans Blue?
1 answer-
This product is rather stable in aqueous solution and may be autoclaved at 15 psi for 30 min. However, dye made up in physiological saline should not be autoclaved.
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