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D2189

Sigma-Aldrich

Recombinant CRM197 expressed in Pseudomonas fluorescens, [Glu52]- Diphtheria toxin

lyophilized powder

Synonym(s):

[Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, CRM 197

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About This Item

CAS Number:
UNSPSC Code:
12352202

form

lyophilized powder

Quality Level

storage temp.

2-8°C

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Packaging

Package size based on protein content

Physical form

Lyophilized powder containing sodium phosphate, sucrose and polysorbate 80

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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F Micoli et al.
Vaccine, 30(5), 853-861 (2011-12-17)
A conjugate vaccine for Salmonella enterica serovar Typhi was produced by chemically linking Vi, purified from Citrobacter, to the non-toxic mutant diphtheria toxin CRM(197) via an adipic dihydrazide spacer using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide coupling chemistry. The polysaccharide purification process was developed based
Naoko Kunami et al.
Anticancer research, 31(7), 2483-2488 (2011-08-30)
The therapeutic outcome for T-cell acute lymphoblastic leukemia (T-ALL) remains poor; thus, novel, targeted therapies are urgently needed. Recently, we showed that heparin-binding epidermal growth factor-like growth factor (HB-EGF), a member of the EGF family, is a promising target for
Fabio Fiorino et al.
Vaccine, 30(43), 6111-6114 (2012-06-19)
Typhoid fever is a public health problem, especially among young children in developing countries. To address this need, a glycoconjugate vaccine Vi-CRM₁₉₇, composed of the polysaccharide antigen Vi covalently conjugated to the non-toxic mutant of diphtheria toxin CRM₁₉₇, is under
Enrico Malito et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(14), 5229-5234 (2012-03-21)
CRM197 is an enzymatically inactive and nontoxic form of diphtheria toxin that contains a single amino acid substitution (G52E). Being naturally nontoxic, CRM197 is an ideal carrier protein for conjugate vaccines against encapsulated bacteria and is currently used to vaccinate
Helen Findlow et al.
The Pediatric infectious disease journal, 31(6), 616-622 (2012-02-16)
From 1999, in the United Kingdom, meningococcal C conjugate (MCC) vaccines from 3 manufacturers were introduced to the infant immunization schedule at 2, 3 and 4 months of age. In 2006, the schedule was refined to a 2-dose primary schedule

Questions

1–4 of 4 Questions  
  1. How do I reconstitute Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 answer
    1. The vial can be reconstituted with 1.0 mL of sterile water. Each vial contains 1 mg of Diphtheria toxin CRM mutant, sodium phosphate buffer, and lactose. It can sometimes be hard to get the product into solution due to the lactose used as a stabilizer. The supplier suggests slight warming (to at least room temperature or 30 °C, but not more than 37 °C) followed by a brief vortex to break up the filaments.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. What is the molecular weight of Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae?

    1 answer
    1. On reducing SDS gels, this protein migrates as a single band of an approximate molecular weight of 63,000 Daltons.

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  4. What kind of mutant, is Product D2189, [Glu52]-Diphtheria toxin from Corynebacterium diphtheriae, and how is this product different from Product No. D0564?

    1 answer
    1. Product No. D0564 is the wild-type toxin and possesses enzymatic activity.  The Glu52 diptheria toxin, also known as CRM197, has a mutation in fragment A (a glycine to glutamic acid substitution at position 52) that results in the complete loss of enzymatic activity. More information on the mutant can be found in the following reference: Uchida, T., Pappenheimer, Jr., A.M. and Harper, A.A.,  J. Biol. Chem., 248, 3851-3854 (1973).

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