BE(2)-M17

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95011816, human nerve, Fusiform

• UNSPSC Code: 41106514

Properties

• biological source: human nerve
• packaging: tube of 5 μg 95011816-DNA-5UG; pkg of vial of cells 95011816-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fusiform
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian neuroblastoma

Cell Line Description

The cell line BE(2)-M17 was isolated from SK-N-BE(2) (Sigma Catalogue number. 95011815) by limiting dilution. The cells show a population doubling time of 20-24 hours, grow in multi-layers but start to aggregate and float as the culture ages. The neuroblastic cells express properties of neuroadrenergic neurones and multiple, moderately long cell processes. BE(2)-M17 showed tyrosine hydroxylase and dopamine-ß-hydroxylase activity and specific uptake of norepinephrine (noradrenalin). Expression of intermediate filaments was detected as well as neuron-specific enolase and moderate expression of vimentin. BE(2)-M17 are tumorigenic in nude mice.

Application

Study of neuroblastoma

Culture Medium

EMEM (EBSS):Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 4x10,000 cells/cm² using 0.1% trypsin or PBS only; 5% CO₂; 37°C.

Other Notes

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