KM703
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
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About This Item
UNSPSC Code:
41106514
biological source
Rat x Mouse Hybridoma
packaging
tube of 5 μg 94090613-DNA-5UG
pkg of vial of cells 94090613-1VL
ImmunoDon
Rat Lou/MN spleen cells
Myeloma
Sp2/0
Reactivity
Anti-Pgp-1, murine homologue of CD44
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Rat Lou/MN spleen x Mouse Sp2/0
Cell Line Description
The hybridoma KM703 was produced by the fusion of Sp2/0 mouse myeloma cells with spleen cells from a Lou/MN rat immunised with the bone marrow derived stromal cell clone, BMS2. The monoclonal antibody recognises an epitope on Pgp-1, the murine homologue of CD44. It prevents the growth of lymphoid or myeloid cells in long term bone marrow cultures.
Immunogen
BUS 2 cells
Application
Determination of ligand specifities and function of Pgp-1/CD44 molecules.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Culture Medium
RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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