DH82
94062922, dog blood, Macrophage
Synonym(s):
DH82 Cell Line
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About This Item
UNSPSC Code:
41106514
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product name
DH82, 94062922
biological source
dog blood
growth mode
Suspension
karyotype
Not specified
morphology
Macrophage
products
Not specified
receptors
FC-gamma receptors
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Canine Monocyte-macrophage
Cell Line Description
Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).
Subculture Routine
Maintain cultures at 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.
Other Notes
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Certificates of Analysis (COA)
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