This cell line is prepared from cultures grown in RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week. Bringing cells out of freeze in a new medium is not recommended. A sudden change of medium may result in poor expansion or viability. The end user would have to determine suitability of another media formulation. In such situations, it is best to transition cells gradually to a new culture medium by adding increasing proportions of the new medium to the old with each split or subculture. See the link below to the ECACC product datasheet for additional information:
https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=93112520&collection=ecacc_gc#
A2780ADR Cell Line human
93112520, human ovary, Epithelial
Synonym(s):
A2780-ADR Cells
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About This Item
UNSPSC Code:
41106514
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Product Name
A2780ADR Cell Line human, 93112520
biological source
human ovary
description
Human Caucasian ovarian carcinoma
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human Caucasian ovarian carcinoma
Cell Line Description
The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (Sigma catalogue no. 93112519) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
Application
Study of drug transport, toxicity testing, drug resistance cancer studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
CSF1PO: 10,11
D13S317: 13
D16S539: 11,14
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 15,16
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
Other Notes
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Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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Hi, I've just purchased the A2780ADR Cell Line human and had a few questions. Would there be any issues with using DMEM media instead of RPMI 1640 media? How is resistance measured in the cells likes and how do I insure that the resistance is retained?
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