HEPM

90120505, human palate epithelium. Established from palatal shelves.

• UNSPSC Code: 41106514

Properties

• Product Name: HEPM, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 90120505
• biological source: human mouth (embryonic)
• packaging: tube of 5 μg 90120505-DNA-5UG; pkg of vial of cells 90120505-1VL
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human embryonic palatal mesenchyme

Cell Line Description

Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.

Application

Proliferation studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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