Derived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm3 using 0.25% trypsin; 5% CO2; 37°C.
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