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C7604

Sigma-Aldrich

Anti-γ-Tubulin-Cy3™ antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-γ-Tubulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

human, chicken

technique(s)

direct immunofluorescence: 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

UniProt accession no.

P23258

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283) , TUBG2(27175)

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General description

γ-Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells.
Tubulin, γ 1 is a protein encoded by the TUBG1 gene in humans. This gene encodes a member of the tubulin superfamily. γ-tubulin interacts with Arp2/3, a complex that nucleates actin filaments and with the Arp2/3 activator WASH. It is found to have relationship with actin filament dynamics.

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Application

Anti-γ- Tubulin-Cy3 antibody produced in rabbit has been used in direct immunofluorescence (IF)and immunofluorescent staining.
Anti-γ-Tubulin-Cy3™ antibody produced in rabbit is suitable for direct immunofluorescence at a dilution of 1:200 using methanol/acetone-fixed chicken fibroblats cells or HeLa cells (human).

Biochem/physiol Actions

γ-Tubulin binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.
γ-Tubulins in the γ-TuRC might promote microtubule nucleation by providing a template that enhances the intrinsically weak lateral interaction between αβ-tubulin heterodimers when associated laterally. It has a unique functional property of a monomeric tubulin. γ-tubulin is also found to be expressed in lung cancer. Cep70 as an important centrosomal protein that interacts with γ-tubulin and plays a critical role in the regulation of mitotic spindle assembly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thermosensitive microgels of poly-N-isopropylacrylamide for drug carriers?Practical approach to synthesis
Musial W, et al.
Acta Poloniae Pharmaceutica, 72, 409-422 (2015)
Widespread GLI expression but limited canonical hedgehog signaling restricted to the ductular reaction in human chronic liver disease
Grzelak CA, et al.
Testing, 12(2), e0171480-e0171480 (2017)
Hector Aldaz et al.
Nature, 435(7041), 523-527 (2005-05-27)
Microtubules are hollow polymers of alphabeta-tubulin that show GTP-dependent assembly dynamics and comprise a critical part of the eukaryotic cytoskeleton. Initiation of new microtubules in vivo requires gamma-tubulin, organized as an oligomer within the 2.2-MDa gamma-tubulin ring complex (gamma-TuRC) of
Xingjuan Shi et al.
The Journal of biological chemistry, 286(38), 33401-33408 (2011-07-29)
Deregulation of the mitotic spindle has been implicated in genomic instability, an important aspect of tumorigenesis and malignant transformation. To ensure the fidelity of chromosome transmission, the mitotic spindle is assembled by exquisite mechanisms and orchestrated by centrosomes in animal
Candice Alexandra Grzelak et al.
PloS one, 12(2), e0171480-e0171480 (2017-02-12)
Canonical Hedgehog (Hh) signaling in vertebrate cells occurs following Smoothened activation/translocation into the primary cilia (Pc), followed by a GLI transcriptional response. Nonetheless, GLI activation can occur independently of the canonical Hh pathway. Using a murine model of liver injury
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Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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