This product is simply a 40% solution of Acrylamide and does not contain any Bis-acrylamide. Note that there are several Acrylamide/Bis-acrylamide 40% solutions available. Please see the link below to review these product options:
https://www.sigmaaldrich.com/substance/acrylamidebisacrylamide40solution1234598765
A4058
Acrylamide solution
40%, suitable for electrophoresis, sterile-filtered
Synonym(s):
2-Propenamide, 40% Acrylamido solution, Acrylic amide, Akrylamid, Polyacrylamide solution
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100 ML
CHF 129.00
5 L
CHF 1’620.00
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100 ML
CHF 129.00
5 L
CHF 1’620.00
About This Item
Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
Beilstein:
6185892
MDL number:
UNSPSC Code:
41105319
eCl@ss:
39031205
PubChem Substance ID:
NACRES:
NB.22
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
concentration
40%
technique(s)
electrophoresis: suitable
storage temp.
2-8°C
SMILES string
NC(=O)C=C
InChI
1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
InChI key
HRPVXLWXLXDGHG-UHFFFAOYSA-N
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General description
Acrylamide has been classified as probably carcinogenic in humans. It is formed in fried and oven-cooked foods due to Maillard reaction between amino acids and reducing sugars.[1]
Application
Acrylamide solution is suitable for use in electrophoresis. It may be used in the preparation of gel buffer for the determination of molecular weight of polypeptide chains by polyacrylamide gel electrophoresis.[2] It may be used in the electrochemical polymerization of pyrrole.[3] It may be used to prepare the pH 7.6 gels, required for the determination of molecular weights of histone by sodium dodecyl sulfate gel electrophoresis.[4]
Preparation Note
Prepared from electrophoresis grade acrylamide in ultra-pure water.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
Target Organs
Peripheral nervous system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Electrochemical polymerization of pyrrole in acrylamide solution.
Sarac AS, et al.
Synthetic Metals, 98(3), 177-182 (1999)
Donald S Mottram et al.
Nature, 419(6906), 448-449 (2002-10-09)
Reports of the presence of acrylamide in a range of fried and oven-cooked foods have caused worldwide concern because this compound has been classified as probably carcinogenic in humans. Here we show how acrylamide can be generated from food components
The molecular weights of vertebrate histones exploiting a modified sodium dodecyl sulfate electrophoretic method.
S Panyim et al.
The Journal of biological chemistry, 246(24), 7557-7560 (1971-12-25)
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
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What is the ratio of acrylamide:bisacrylamide in this product? Is it 37.5:1, 29:1 or 19:1 (or maybe other?)
1 answer-
Helpful?
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What is the solvent in this solution. Water? Or organic solvent?
1 answer-
The solution is prepared from electrophoresis grade acrylamide in ultra-pure water and aseptically filtered to insure the highest quality and reproducibility.
Helpful?
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