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A3054

Sigma-Aldrich

Agarose

For pulsed field electrophoresis sample preparation

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan

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About This Item

CAS Number:
9012-36-6
EC Number:
232-731-8
MDL number:
MFCD00081294
UNSPSC Code:
12352201
PubChem Substance ID:
24890482
NACRES:
NA.21

biological source

algae (red)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤10% water

EEO

≤0.10

transition temp

gel point ≤30 °C

anion traces

sulfate (SO42-): ≤0.15%

foreign activity

DNase and RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Application

Especially formulated for preparing chromosomal DNA samples prior to pulsed-field gel electrophoresis (PFGE). Making gel plugs for the separation of high molecular weight DNA helps prevent shearing encountered during conventional isolation. Cells can be lysed and the DNA digested with restriction endonucleases while imbedded in agarose plugs.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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