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30326

Sigma-Aldrich

EMPIGEN® BB detergent

~30% active substance

Synonym(s):

N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine

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50 ML
CHF 60.30
250 ML
CHF 132.00

CHF 60.30

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50 ML
CHF 60.30
250 ML
CHF 132.00

About This Item

Linear Formula:
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
CAS Number:
66455-29-6
Beilstein:
3670807
EC Number:
266-368-1
MDL number:
MFCD00084742
UNSPSC Code:
12161900
PubChem Substance ID:
329753464
NACRES:
NA.25

CHF 60.30

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description

zwitterionic

Quality Level

200

form

liquid

composition

solid content, 35.0-45.0 % (w/v)

concentration

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

density

1.030-1.060 g/mL at 20 °C

UV absorption

λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037

SMILES string

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

InChI key

DVEKCXOJTLDBFE-UHFFFAOYSA-N

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Application

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity[1]. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease[2]. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses[3].

Legal Information

EMPIGEN is a registered trademark of Huntsman Corporation

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH5993
BCBQ6339V
BCBQ6339
BCBP8409

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Kulbir Singh et al.
Langmuir : the ACS journal of surfaces and colloids, 30(13), 3673-3680 (2014-03-15)
In this article, we report additive-induced micellar growth and rheology modification for mixtures of anionic (sodium dodecyl sulfate, SDS) and zwitterionic (N-alkylated glycine derivative, Empigen BB or EBB) surfactants. Two nonaromatic hydrotropic salts (hexyltrimethylammonium bromide, C6TAB, and/or dibutylenebis(dimethylbutylammonium bromide), 4-4-4)
L A Lowthert et al.
Biochemical and biophysical research communications, 206(1), 370-379 (1995-01-05)
Intermediate filament (IF) proteins make up some of the most insoluble proteins known, and within the IF protein family, keratins are the least soluble. We compared the efficiency of nonionic, cationic, mixed nonionic and anionic, and zwitterionic detergents in solubilizing
James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
Islam Khan et al.
Microbes and infection, 7(5-6), 853-859 (2005-05-10)
Campylobacter jejuni is a major food-borne pathogen and a leading cause of diarrhoea. A cytotoxin is most likely involved in the pathogenesis of inflammatory diarrhoea due to C. jejuni. A 45-kDa outer membrane protein encoded by the porA gene was
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. 이 용액은 증류수에 30% 희석되어 있는 건가요? 아니라면 희석한 용액(active substance)이 무엇인지 궁금합니다.

    1 answer

    1. This product is a 30% solution in water. The active substances are chemically identified as C12-14-Alkyldimethyl-betaines. Please see the link below to review the product Safety Data Sheet:
      https://www.sigmaaldrich.com/sds/sigma/30326

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