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12106C

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonym(s):

HI FBS, HI FCS, HI Fetal Bovine Sera, HI Fetal Bovine Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Fetal Bovine Serum (FBS) provides many non-defined growth promoting and survival enhancing factors to cells in culture. FBS, heat inactivated may be used in place of regular FBS wherein the inactivation of the complement system is beneficial to cell growth or function. Heat-inactivated FBS is used to culture Leishmania sp.

Preparation Note

Prepared by heating at 56°C for 30 minutes.
Prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes is used to inactivate the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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E Anitua et al.
Experimental eye research, 119, 27-34 (2013-12-19)
We have developed and characterized a new type of plasma rich in growth factors (PRGF) derived eye-drop therapy for patients suffering from autoimmune diseases. To determine the concentration of several growth factors, proteins, immunoglobulins and complement activity of the heat-inactivated
Nand Lal Sharma et al.
The American journal of tropical medicine and hygiene, 72(6), 819-824 (2005-06-21)
Localized cutaneous leishmaniasis (LCL) in India is due mostly to Leishmania tropica. It is mainly endemic in the deserts of Rajasthan. Recently, Himachal Pradesh has been identified as a new endemic focus for the disease. In the last few years
Perrine Barraud et al.
BMC neuroscience, 9, 24-24 (2008-02-20)
The olfactory epithelium is a neurogenic tissue comprising a population of olfactory receptor neurons that are renewed throughout adulthood by a population of stem and progenitor cells. Because of their relative accessibility compared to intra-cranially located neural stem/progenitor cells, olfactory
Robert C Tuckey et al.
The Journal of steroid biochemistry and molecular biology, 112(4-5), 213-219 (2008-11-13)
Cytochrome P450scc (CYP11A1) metabolizes vitamin D3 to 20-hydroxyvitamin D3 as the major product, with subsequent production of dihydroxy and trihydroxy derivatives. The aim of this study was to determine whether cytochrome P450scc could metabolize 1alpha-hydroxyvitamin D3 and whether products were
Thi A Tran et al.
Journal of neuroinflammation, 5, 14-14 (2008-05-14)
Recent animal and human studies implicate chronic activation of microglia in the progressive loss of CNS neurons. The inflammatory mechanisms that have neurotoxic effects and contribute to neurodegeneration need to be elucidated and specifically targeted without interfering with the neuroprotective
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Protocols

WST-1 Assay Protocol for Cell Viability

WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.

Cell Viability and Proliferation XTT Assay Protocol Guide

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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