Skip to Content
Merck
All Photos(2)

Documents

90107

Sigma-Aldrich

Gram′s iodine solution

for microscopy

Synonym(s):

Potassium Triiodide

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

100

product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

color

brown to very dark brown

refractive index

n20/D 1.335

density

1.005 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

bacteria

Related Categories

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM2491
BCCM2490
BCCM2489
BCCK4652
BCCK4651

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Practical Methods for Environmental Microbiology and Biotechnology (2002)

Articles

Campylobacter Detection and Identification

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service