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48305

Millipore

Bile salts

suitable for microbiology

Synonym(s):

Bile acids sodium salt, cholic acid-deoxycholic acid sodium salt mixture, Bile acids sodium salt, Cholic acid-Deoxycholic acid sodium salt mixture

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About This Item

MDL number:
MFCD00130648
UNSPSC Code:
41106212
NACRES:
NA.85

description

anionic

Quality Level

200

sterility

non-sterile

Assay

40-60% (Cholic Acid ; approximately 50% sodium cholate and approximately 50% sodium deoxycholate, HPLC)

form

powder

composition

cholic acid sodium salt, ~50%
deoxycholic acid sodium salt, ~50%

impurities

≤5% water

loss

≤5% loss on drying (water)

solubility

H2O: 0.1 g/mL, clear, faintly yellow

application(s)

microbiology

storage temp.

10-30°C

Related Categories

General description

Bile salt is an organic sodium salt with a conjugate of any of the bile acids along with either glycine or taurine. Biles salts are composed of four different kinds of bile acids - cholic, deoxycholic, chenodeoxycholic and lithocholic acids.
Bile salts are extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media, where it behaves as a selective inhibitory agent that enables isolation and identification of pathogens. For example, in MacConkey Agar/Broth.

Application

Bile salts may be used as one of the selective agents to prevent the growth of injured Escherichia coli cells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injured Escherichia coli ML30 cells. It may also be used for identification of isolates of Vibrio (Beneckea) vulnificus which is a halophilic bacterium, found responsible for causing several human infections.

Biochem/physiol Actions

Bile salts are surface-active steroid compounds and their primary physiological function is to aid in the digestion of lipophilic nutrients in intestinal tracts of vertebrates.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK4542
BCCH7185
BCCJ5649
BCCJ5529
BCCG0220

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Bacterial degradation of bile salts.
Philipp B
Applied Microbiology and Biotechnology, 89 (4), 903-915 (2011)
Bonggyu Min et al.
Frontiers in bioengineering and biotechnology, 8, 828-828 (2020-08-15)
Stress resistance is an important trait expected of lactic acid bacteria used in food manufacturing. Among the various sources of stress, high temperature is a key factor that interrupts bacterial growth. In this regards, constant efforts are made for the
Eunsil Choi et al.
Frontiers in microbiology, 11, 597515-597515 (2020-11-27)
The BipA (BPI-inducible protein A) protein is ubiquitously conserved in various bacterial species and belongs to the translational GTPase family. Interestingly, the function of Escherichia coli BipA is not essential for cell growth under normal growth conditions. However, cultivation of
Luis Noriega et al.
International journal of food microbiology, 94(1), 79-86 (2004-06-03)
Six derivatives with increased resistance to ox gall (MIC: > or = 1% w/v) and one derivative resistant to sodium cholate (MIC: 0.8% w/v) were obtained from more sensitive original Bifidobacterium strains. These microorganisms, and two additional cholate resistant derivatives
D L Scheusner et al.
Applied microbiology, 21(1), 46-49 (1971-01-01)
A population of Escherichia coli ML30 cells was exposed to a quaternary ammonium compound, and injury to the cells was measured by a comparison of counts on Trypticase Soy Agar and Violet Red Bile Agar. Substantial injury could not be
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Articles

Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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