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2-NP-AHD

VETRANAL®, analytical standard

Synonym(s):

1-(2-Nitrobenzylidenamino)-2,4-imidazolidinedione

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About This Item

Empirical Formula (Hill Notation):
C10H8N4O4
CAS Number:
Molecular Weight:
248.19
MDL number:
UNSPSC Code:
41116107
NACRES:
NA.24

grade

analytical standard

Quality Level

product line

VETRANAL®

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

clinical testing

format

neat

SMILES string

O=C(C1)NC(N1/N=C/C2=C([N+]([O-])=O)C=CC=C2)=O

InChI

1S/C10H8N4O4/c15-9-6-13(10(16)12-9)11-5-7-3-1-2-4-8(7)14(17)18/h1-5H,6H2,(H,12,15,16)/b11-5+

InChI key

FULJCJZKZXOFQZ-VZUCSPMQSA-N

General description

2-NP-AHD belongs to the class of nitrofurans (NFs), a series of synthetic broad spectrum antibiotics, used for veterinary applications.
2-NP-AHD is a metabolite of nitrofurans type of antibiotics generally used as veterinary drugs.

Application

2-NP-AHD may be used as an analytical reference standard for the determination of the analyte in shrimp and aquatic products using ultra performance liquid chromatography(UPLC) and liquid chromatography(LC) coupled to tandem mass spectrometry (MS/MS).
2-NP-AHD may be used as an analytical standard for the determination of 2-NP-AHD in:
  • Honey by modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction followed by liquid chromatography-tandem spectrometry (LC-MS/MS) with multiple reaction monitoring (MRM) detection and visualized microarray screen assay.
  • Animal-derived foods by water-compatible molecularly imprinted solid-phase extraction (SPE) and high performance LC with MS/MS.
  • Katla fish (Katla katla) and Bagda shrimp (Penious monodon) by ultra-performance LC-MS/MS.

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Other Notes

Derivatized standard for the assay of nitrofurans in animal tissue by LC/MS

Recommended products

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

Legal Information

VETRANAL is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Development of water-compatible molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry for the detection of six sulfonamides in animal-derived foods.
Zhao X, et al.
Journal of Chromatography A, 1574, 9-17 (2018)
Determination of Nitrofuran Metabolites in Shrimp by LC/MS/MS.
Ye L, et al.
Laboratory Information Bulletin, 4466, 1-20 (2011)
Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay.
Zhoumin L, et al.
Food Chemistry, 190, 982-989 (2017)
A SIMULTANEOUS METHOD DEVELOPMENT, VALIDATION AND CONFIRMATORY ANALYSIS OF AMOZ, AOZ, AHD AND SEM IN KATLA FISH (KATLA KATLA) AND BAGDA SHRIMP (PENIOUS MONODON) BY UPLC-MS/MS (QPXE).
Alam MA and Muhammad T
Journal of Biotechnology, 2(3), 102-112 (2014)
Sara E McNamee et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 34(4), 562-572 (2017-01-13)
Practical solutions for multiple antibiotic determination in food are required by the food industry and regulators for cost-effective screening purposes. This study describes the feasibility in development and preliminary performance of a novel multispot nanoarray for antibiotic screening in honey.

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