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Supelco

Esculin hydrate

analytical standard

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About This Item

Empirical Formula (Hill Notation):
C15H16O9 · xH2O
CAS Number:
531-75-9
Molecular Weight:
340.28 (anhydrous basis)
EC Number:
207-646-4
MDL number:
MFCD00149492
UNSPSC Code:
85151701
PubChem Substance ID:
329748219
NACRES:
NA.24

grade

analytical standard

Quality Level

100

Assay

≥98.0% (HPLC)

optical activity

[α]/D -40.5±2.5°, c = 5% in pyridine

shelf life

limited shelf life, expiry date on the label

impurities

≤11% water

application(s)

food and beverages

format

neat

SMILES string

O.OC[C@H]1O[C@@H](Oc2cc3C=CC(=O)Oc3cc2O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C15H16O9.H2O/c16-5-10-12(19)13(20)14(21)15(24-10)23-9-3-6-1-2-11(18)22-8(6)4-7(9)17;/h1-4,10,12-17,19-21H,5H2;1H2/t10-,12-,13+,14-,15-;/m1./s1

InChI key

CQYPGSKIFJFVDQ-QWFKVUSTSA-N

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Application

Esculin hydrate may be used as an analytical standard for:
  • The detection of glucosidase inhibitors by thin-layer chromatography (TLC) bioautographic method.
  • Library screening in the characterization of novel cellulase genes from uncultured microorganisms.
  • Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin.
  • Detecting β-glucosidase activity in the identification of novel cellulase genes from uncultured microorganisms in rabbit cecum.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD9754
BCBX3936
BCBQ9874V
BCBL3493V

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Yi Feng et al.
Applied microbiology and biotechnology, 75(2), 319-328 (2007-01-12)
A metagenomic cosmid library was prepared in Escherichia coli from DNA extracted from the contents of rabbit cecum and screened for cellulase activities. Eleven independent clones expressing cellulase activities (four endo-beta-1,4-glucanases and seven beta-glucosidases) were isolated. Subcloning and sequencing analysis
Fengchao Wang et al.
Microbiological research, 164(6), 650-657 (2009-02-24)
Four different environmental DNA libraries were prepared from microbial consortium collected from forest soil, dung of elephant, cow rumen and rotted tree. Seven independent clones specifying cellulase activities (five endo-beta-1,4-glucanases and two beta-glucosidases) were isolated and identified. Sequence analysis of
Mario O Salazar et al.
Phytochemical analysis : PCA, 18(3), 209-212 (2007-05-16)
A new bioautographic assay suitable for the localisation of beta-glucosidase inhibitors present in a complex matrix is described. Enzyme activity was detected using esculin as the substrate to produce esculetin, which reacts with ferric ion to form a brown complex.
Ghada Ben Rhouma et al.
Food chemistry, 135(3), 1700-1707 (2012-09-08)
Rutin and esculin have been polymerised by laccase. Five fractions with M(w)¯ between 2127.42 and 8331.85g/mol for oligorutins, and between 688.12 and 6973g/mol for oligoesculins, were obtained. Fourier transformed infrared analysis showed that oligorutins were formed through C-C, C-O and
I Diezhandino et al.
Food chemistry, 168, 134-141 (2014-08-31)
The aim of this work was to study the microbiological, physico-chemical and proteolytic changes in Valdeón blue-veined cheese during ripening. Eight replicas of cheese were produced and a total of 48 cheeses were analysed. Lactic acid bacteria, mainly lactococci, were
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