11814460001
Roche
Anti-GFP
from mouse IgG1κ (clones 7.1 and 13.1)
Synonym(s):
anti-green fluorescent protein
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About This Item
UNSPSC Code:
12352203
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
13.1, monoclonal
7.1, monoclonal
Assay
>90% (HPLC)
form
lyophilized
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG1κ
storage temp.
2-8°C
Related Categories
General description
Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFP′s green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.
Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.
Specificity
Subtype: Both clones are Mouse IgG1κ
Application
Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using:
- Immunoprecipitation
- Western blots
- Immunostaining
Features and Benefits
Contents
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200μg of total Anti-GFP IgG.
Anti-GFP is a mixture of two clones (7.1 and 13.1).
Quality
Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate.
The following concentrations should be taken as a guideline:
Storage conditions (working solution): -15 to -25 °C
The following concentrations should be taken as a guideline:
- Western blot: 1:1000 dilution
- Immunoprecipitation: 2 to 10 μg
Storage conditions (working solution): -15 to -25 °C
Reconstitution
Add 500 μl double distilled water to a final concentration of 0.4 mg/ml.
Rehydrate on ice for 30 minutes.
Rehydrate on ice for 30 minutes.
Legal Information
This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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