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11417991001

Roche

SuRE/Cut™ Buffer H

solution, pkg of 5 × 1 mL

Synonym(s):

SuRE/Cut Buffer for Restriction Enzymes, restriction enzyme buffer

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About This Item

UNSPSC Code:
41105600

form

solution

Quality Level

100

packaging

pkg of 5 × 1 mL

manufacturer/tradename

Roche

storage temp.

−20°C

Related Categories

General description

The SuRE/Cut Buffer System consists of five optimized incubation buffers, 10x concentrated, for DNA restriction digest. Activity of all restriction enzymes has been determined in each buffer to select 100% activity or to calculate activity in double-digests.


Application

SuRE/Cut™ Buffer H has been used to treat EcoRI and XhoI restriction enzymes to digest gel-purified reaction products and the pMX-IG vector. It has also been used to dilute the restriction enzyme Xanthomonas badrii I for the digestion of the genomic DNA of the E. coli colonies and the Salmonella Braenderup H2812 (ATCC BAA-664) marker.
The SuRE/Cut Buffer H has been used for digestion of genomic DNA using the restriction enzyme XbaI. It has been used for digestion of PCR products and plasmid using EcoRI and XhoI.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

SuRE/Cut is a trademark of Roche

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificates of Analysis (COA)

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Amber E Alsop et al.
Nature communications, 6, 6841-6841 (2015-04-17)
During apoptosis, Bak permeabilizes mitochondria after undergoing major conformational changes, including poorly defined N-terminal changes. Here, we characterize those changes using 11 antibodies that were epitope mapped using peptide arrays and mutagenesis. After Bak activation by Bid, epitopes throughout the
Giel Vandemoortele et al.
Bio-protocol, 7(7), e2211-e2211 (2017-04-05)
The programmable Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease 9 (Cas9) technology revolutionized genome editing by providing an efficient way to cut the genome at a desired location (Ledford, 2015). In mammalian cells, DNA lesions trigger the error-prone non-homologous
W J M Landman et al.
Avian pathology : journal of the W.V.P.A, 43(4), 345-356 (2014-06-20)
Escherichia coli colonies isolated from the bone marrow of fresh dead hens of laying flocks with the E. coli peritonitis syndrome (EPS) were genotyped using pulsed-field gel electrophoresis (PFGE). Typing is important from an epidemiological point of view and also

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Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

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Restriction Enzymes

Restriction endonucleases in prokaryotes function primarily to protect against foreign genetic material, notably bacteriophage DNA.

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