MAB5556
Anti-Nicastrin Antibody
ascites fluid, clone 9C3, Chemicon®
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
9C3, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NCSTN(23385)
Specificity
Nicastrin.
Immunogen
Synthetic peptide corresponding to the C terminal 18 a.a. of mouse Nicastrin. Due to sequence similarity, it is expected that the antibody will react with most mammals.
Application
Anti-Nicastrin Antibody is an antibody against Nicastrin for use in IP, WB & IC.
Western blot. The antibody recognizes a doublet of ~100-110 kDa. Suggested antibody dilution buffer is TBS with 0.1% Tween-20 and 5% non-fat milk powder. Suggested incubation time is overnight at 2-8°C or 1-2 hours at room temperature.
Immunocytochemistry
Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Jolanta L Lundgren et al.
Journal of neurochemistry, 135(3), 606-615 (2015-08-25)
Synaptic degeneration and accumulation of the neurotoxic amyloid β-peptide (Aβ) in the brain are hallmarks of Alzheimer disease. Aβ is produced by sequential cleavage of the amyloid precursor protein (APP), by the β-secretase β-site APP cleaving enzyme 1 (BACE1) and
Mitsuhiro Inoue et al.
The FEBS journal, 282(14), 2587-2599 (2015-04-22)
The transmembrane protease complex γ-secretase is a key enzyme in Alzheimer disease pathogenesis as it liberates the neurotoxic amyloid β-peptide (Aβ); however, the mechanism of regulation of its activity in various cell types and subcellular compartments is largely unknown. Several
Yasuhiro Teranishi et al.
The FEBS journal, 282(17), 3438-3451 (2015-06-23)
γ-Secretase is a transmembrane protease complex that is responsible for the processing of a multitude of type 1 transmembrane proteins, including the amyloid precursor protein and Notch. γ-Secretase processing of amyloid precursor protein results in the release of the amyloid
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Rocío Pérez-González et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9), 12922-12931 (2020-08-11)
Pleiotropic roles are proposed for brain extracellular vesicles (EVs) in the development of Alzheimer's disease (AD). Our previous studies have suggested a beneficial role for EVs in AD, where the endosomal system in vulnerable neurons is compromised, contributing to the
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service