HTS084RTA
Ready-to-Assay BB 2 Bombesin Receptor Frozen Cells
Human
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
General description
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B. A family of 3 GPCRs, including NMB-R (BB1), GRP-R (BB2) and BRS-3 (BB3), mediate the biological effects of the peptides. The receptors differ in their affinities for the peptides; BB2 binds to GRP with 50-300-fold greater affinity than to NMB, whereas BB1 binds to NMB with 10-800-fold greater affinity than to GRP (Tokita et al., 2004). Binding of ligand to BB2 activates Gq to increase intracellular calcium concentrations. GRP stimulates release of gastrin from endocrine cells and stimulates smooth muscle activity in the gastrointestinal tract. In addition, binding of GRP to BB2 stimulates proliferation of a variety of cell types, and has been implicated in the progression of small cell lung cancer and other malignancies. The CNS is also a major site of GRP expression, and GRP and BB2 are involved in the circadian clock, conditioned fear, and food intake (Ohki-Hamazaki et al., 2005). Millipore′s cloned human BB2 expressing cell line is made in the Chem-1 host, which supports high levels of recombinant BB2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at BB2.
Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B. A family of 3 GPCRs, including NMB-R (BB1), GRP-R (BB2) and BRS-3 (BB3), mediate the biological effects of the peptides. The receptors differ in their affinities for the peptides; BB2 binds to GRP with 50-300-fold greater affinity than to NMB, whereas BB1 binds to NMB with 10-800-fold greater affinity than to GRP (Tokita et al., 2004). Binding of ligand to BB2 activates Gq to increase intracellular calcium concentrations. GRP stimulates release of gastrin from endocrine cells and stimulates smooth muscle activity in the gastrointestinal tract. In addition, binding of GRP to BB2 stimulates proliferation of a variety of cell types, and has been implicated in the progression of small cell lung cancer and other malignancies. The CNS is also a major site of GRP expression, and GRP and BB2 are involved in the circadian clock, conditioned fear, and food intake (Ohki-Hamazaki et al., 2005). Millipore′s cloned human BB2 expressing cell line is made in the Chem-1 host, which supports high levels of recombinant BB2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at BB2.
Cell Line Description
- GPCR Cell Lines
- Host cells: Chem-1
Application
Human BB2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Biochem/physiol Actions
- GPCR Class: A
- Protein Target: BB2
- Target Sub-Family: Bombesin
Components
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Disclaimer
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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