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FCMAB306P

Sigma-Aldrich

Milli-Mark Anti-Human-Nuclei-PE Antibody, clone 235-1

clone 235-1, Milli-Mark®, from mouse

Synonym(s):

HuNu

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

235-1, monoclonal

species reactivity (predicted by homology)

human (based on 100% sequence homology)

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

General description

Stem-cell and fetal-cell replacement therapies for many diseases have proven to be very hopeful. Human nuclei antibodies are very important histological and cytological markers for identifying human cells. The use of human nuclei and C-peptide to force differentiation of human mesenchymal stem cells into insulin-producing cells to treat diabetes mellitus in mice has been successful. Human nuclei cells have also been associated with using stem-cell treatment in spinal cord injuries in canines.

Specificity

Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.

Application

Detect Human-Nuclei using this Milli-Mark Anti-Human-Nuclei-PE Antibody, clone 235-1 validated for use in FC.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells

Quality

Evaluated by flow cytometry using Jurkat cells.

Physical form

Protein A purified
Purified mouse monoclonal IgG1κ conjugated to PE in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Jurkat cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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