Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Specificity
Broad species cross-reactivity is expected based on sequence homology.
Immunogen
Epitope: methylated lysine 9
KLH-conjugated, synthetic 2X-branched peptide corresponding to histone H3 monomethylated at lysine 9.
Application
Detect monomethyl-Histone H3 (Lys9) using this Milli-Mark Anti-monomethyl-Histone H3 (Lys9)-FITC Antibody validated for use in FC.
Research Category Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category Histone Modifying Proteins
Histones
Quality
Evaluated by flow cytometry using HeLa cells.
Target description
16 kDa Calculated
Physical form
Protein A purified
Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control HeLa cells
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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