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form
powder
mol wt
Mw 681 Da
manufacturer/tradename
Fluoro-Jade®
technique(s)
immunofluorescence: suitable
fluorescence
λex 480 nm; λem 525 nm
General description
Fluoro-JadeⓇ B is a polyanionic fluorescein derivative which sensitively and specifically binds to degenerating neurons. It is a dark red power that has a green iridescence with excitation peak at 480 nm and emission peak at 525 nm. The filter used for visualizing Fluoro-JadeⓇ B is a fluorescein/FITC filter. Fluoro-JadeⓇ B can be used on most tissue section types and thicknesses including frozen, vibratomed cryostat or paraffin-embedded sections from 3-50 µm. Fluoro-JadeⓇ B is faster and more reliable than older methods (e.g. suppressed silver) for the unequivocal qualitative detection and quantitative measurement of both and fine scale neuronal degeneration.
N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells
Application
Appearance: Dark red powder with green iridescence.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Warning
Toxicity: Although the compound appears to be of low toxicity, it has not been extensively evaluated and therefore routine laboratory caution should be exercised. Not intended for human consumption.
Physical form
Filter System : Fluorescein / FITC
Solubility: Very soluble in water and bases, moderately soluble in alcohol and weak acids.
Storage and Stability
The powder should be stored well sealed at room temperature, preferable in a dessicator, due to its hygroscopic nature. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The working solution (0.0004%) in 0.1% acetic acid should be prepared fresh and not be stored or reused.
Analysis Note
This layer chromatography using cellulose plates and a solvent system of n-propinol, water, and ammonium hydroxide (6:5:2) revealed the presence of two fluorescent isomers and two trace non-fluorescent bands. No amount of fluorescein or Fluoro-Jade was present
Legal Information
FLUORO-JADE is a registered trademark of Histo-Chem, Inc.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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