71353-M

Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen

Escherichia coli, rod shaped

• UNSPSC Code: 41106202
• NACRES: NA.81

Properties

• product name: Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen, Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
• biological source: Escherichia coli
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: transformation efficiency: 2×10⁶ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac⁺ lacI^q pro] gor522::Tn10 trxB pLacIRARE2 (Cam^R, Str^R, Tet^R)





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Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. In Rosetta-gami 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami 2(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany