Skip to Content
Merck
All Photos(1)

Documents

69670-M

Millipore

His•Bind® Resin

Synonym(s):

uncharged IDA agarose beads

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

form

liquid

manufacturer/tradename

Novagen®

storage condition

do not freeze

application(s)

life science and biopharma

storage temp.

2-8°C

General description

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Application

His•Bind® Resin have been used: in purification of His•Tag® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndromein protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.

Biochem/physiol Actions

His•Bind® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the His•Bind® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the His•Bind® resin. The target protein can be easily recovered by eluting with imidazole. The His•Bind® Resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Features and Benefits

  • Reusable many times
  • Compatible with His•Bind® Buffer Kit
  • Compatible with 1 mM THP

Warning

Toxicity: Standard Handling (A)

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

86.0 °F

Flash Point(C)

30 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The human RAP1 and GFAP? proteins increase ?-secretase activity in a yeast model system
Swanson MJ, et al.
G3: Genes, Genomes, Genetics, 13(8) (2023)
Anaerobic phloroglucinol degradation by Clostridium scatologenes
Zhou Y, et al.
Environmental Microbiology, 14(4) (2023)
Heterogeneity in Lowe Syndrome: Mutations Affecting the Phosphatase Domain of OCRL1 Differ in Impact on Enzymatic Activity and Severity of Cellular Phenotypes
J Lee J, et al.
Biomolecules, 13(4), 615-615 (2023)

Articles

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service